New Research


2009/07/01

Realtime CARS microscopy

-Visualization of specific molecules without staining-

Professor Tsutomu ARAKI, Associate Professor Mamoru HASHIMOTO, Assistant Professor Takeshi YASUI, As

The aims of Biomedical Photonics Group (Araki Lab.) are contributions to medical diagnostics or biomedical researches by instrumentation utilizing new principles, new methods, and so on. Last year\'s Nobel Prize in Chemistry was awarded for “the discovery and development of Green Fluorescent Protein, GFP”. Researchers can now connect GFP to other interesting proteins using DNA technology. However, connection of GFP to other proteins sometimes disturbs the original function of the proteins.
Therefore, new visualization method without any chemical and/or physical preparation is required. For that purpose, use of coherent anti-Stokes Raman scattering (CARS) is attractive, which is one of the nonlinear optical phenomena.
We have developed CARS microscopy system using two highly synchronized picosecond lasers and a high scanning rate multi beam scanner, and have realized the realatime molecular vibrational imaging. Any molecule has molecular vibrations and the frequencies of molecular vibrations are very sensitive to molecular spices. The figure shows the resultant image of lipid molecules in cancer cells (three-dimensionally reconstructed).


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